Safer non-viral DNA delivery using lipid nanoparticles loaded with endogenous anti-inflammatory lipids – Nature Biotechnology

May Be Interested In:Future negotiations with Hamas 'will take place only under fire', says Netanyahu


  • Tenchov, R., Bird, R., Curtze, A. E. & Zhou, Q. Lipid nanoparticles—from liposomes to mRNA vaccine delivery, a landscape of research diversity and advancement. ACS Nano 15, 16982–17015 (2021).

  • Hou, X., Zaks, T., Langer, R. & Dong, Y. Lipid nanoparticles for mRNA delivery. Nat. Rev. Mater. 6, 1078–1094 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Pardi, N. et al. Expression kinetics of nucleoside-modified mRNA delivered in lipid nanoparticles to mice by various routes. J. Control. Release 217, 345–351 (2015).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Herweijer, H. et al. Time course of gene expression after plasmid DNA gene transfer to the liver. J. Gene Med. 3, 280–291 (2001).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Das, A. T., Tenenbaum, L. & Berkhout, B. Tet-On systems for doxycycline-inducible gene expression. Curr. Gene Ther. 16, 156–167 (2016).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Arjomandnejad, M., Dasgupta, I., Flotte, T. R. & Keeler, A. M. Immunogenicity of recombinant adeno-associated virus (AAV) vectors for gene transfer. BioDrugs 37, 311–329 (2023).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Bulcha, J. T., Wang, Y., Ma, H., Tai, P. W. L. & Gao, G. Viral vector platforms within the gene therapy landscape. Signal Transduct. Target. Ther. 6, 53 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Zhu, Y. et al. Multi-step screening of DNA/lipid nanoparticles and co-delivery with siRNA to enhance and prolong gene expression. Nat. Commun. 13, 4282 (2022).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Guimaraes, L. C. et al. Nanoparticle-based DNA vaccine protects against SARS-CoV-2 variants in female preclinical models. Nat. Commun. 15, 590 (2024).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Liao, H.-C. et al. Lipid nanoparticle-encapsulated DNA vaccine robustly induce superior immune responses to the mRNA vaccine in Syrian hamsters. Mol. Ther. Methods Clin. Dev. 32, 101169 (2024).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Nguyen, T. N. et al. Lipid nanoparticle-encapsulated DNA vaccine confers protection against swine and human-origin H1N1 influenza viruses. mSphere 9, e0028324 (2024).

    Article 
    PubMed 

    Google Scholar 

  • Yu, L. & Liu, P. Cytosolic DNA sensing by cGAS: regulation, function, and human diseases. Signal Transduct. Target. Ther. 6, 170 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Cheng, Z. et al. The interactions between cGAS-STING pathway and pathogens. Signal Transduct. Target. Ther. 5, 91 (2020).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Hansen, A. L. et al. Nitro-fatty acids are formed in response to virus infection and are potent inhibitors of STING palmitoylation and signaling. Proc. Natl Acad. Sci. USA 115, E7768–E7775 (2018).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Pardi, N. et al. Administration of nucleoside-modified mRNA encoding broadly neutralizing antibody protects humanized mice from HIV-1 challenge. Nat. Commun. 8, 14630 (2017).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Chase, L. S., Zaleski, M. H., Morrell, L. J. & Brenner, J. S. Automated measurement of distance-walked as a ‘sixth vital sign’ for detecting infusion reactions during preclinical testing. Int. J. Pharm. 645, 123369 (2023).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Domizio, J. D. et al. The cGAS–STING pathway drives type I IFN immunopathology in COVID-19. Nature 603, 145–151 (2022).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Zhang, X., Goel, V. & Robbie, G. J. Pharmacokinetics of patisiran, the first approved RNA interference therapy in patients with hereditary transthyretin-mediated amyloidosis. J. Clin. Pharmacol. 60, 573–585 (2020).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Schoenmaker, L. et al. mRNA-lipid nanoparticle COVID-19 vaccines: structure and stability. Int. J. Pharm. 601, 120586 (2021).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Parhiz, H. et al. Added to pre-existing inflammation, mRNA-lipid nanoparticles induce inflammation exacerbation (IE). J. Control. Release 344, 50–61 (2022).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Banete, A., Barilo, J., Whittaker, R. & Basta, S. The activated macrophage—a tough fortress for virus invasion: how viruses strike back. Front. Microbiol. 12, 803427 (2022).

  • Sasaki, A. & Kinjo, M. Monitoring intracellular degradation of exogenous DNA using diffusion properties. J. Control. Release 143, 104–111 (2010).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Wang, D., Zhao, H., Shen, Y. & Chen, Q. A variety of nucleic acid species are sensed by cGAS, implications for its diverse functions. Front. Immunol. 13, 826880 (2022).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Melo, T., Montero-Bullón, J.-F., Domingues, P. & Domingues, M. R. Discovery of bioactive nitrated lipids and nitro-lipid-protein adducts using mass spectrometry-based approaches. Redox Biol. 23, 101106 (2019).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Khoo, N. K. H., Li, L., Salvatore, S. R., Schopfer, F. J. & Freeman, B. A. Electrophilic fatty acid nitroalkenes regulate Nrf2 and NF-κB signaling: a medicinal chemistry investigation of structure-function relationships. Sci. Rep. 8, 2295 (2018).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Kaminski, J. J. et al. Synthetic oligodeoxynucleotides (ODN) containing suppressive TTAGGG motifs inhibit AIM2 inflammasome activation. J. Immunol. 191, 3876–3883 (2013).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Tao, R. et al. Long-term metabolic correction of phenylketonuria by AAV-delivered phenylalanine amino lyase. Mol. Ther. Methods Clin. Dev. 19, 507–517 (2020).

    Article 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Sperinde, J. J., Choi, S. J. & Szoka, F. C. Phage display selection of a peptide DNase II inhibitor that enhances gene delivery. J. Gene Med. 3, 101–108 (2001).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Bai, H., Lester, G. M. S., Petishnok, L. C. & Dean, D. A. Cytoplasmic transport and nuclear import of plasmid DNA. Biosci. Rep. 37, BSR20160616 (2017).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Cheng, Q. et al. Selective organ targeting (SORT) nanoparticles for tissue-specific mRNA delivery and CRISPR–Cas gene editing. Nat. Nanotechnol. 15, 313–320 (2020).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Wei, T. et al. Lung SORT LNPs enable precise homology-directed repair mediated CRISPR/Cas genome correction in cystic fibrosis models. Nat. Commun. 14, 7322 (2023).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Omo-Lamai, S. et al. Physicochemical targeting of lipid nanoparticles to the lungs induces clotting: mechanisms and solutions. Adv. Mater. 36, e2312026 (2024).

    Article 
    PubMed 

    Google Scholar 

  • Zaleski, M. H. et al. Nanocarriers’ repartitioning of drugs between blood subcompartments as a mechanism of improving pharmacokinetics, safety, and efficacy. J. Control. Release 374, 425–440 (2024).

    Article 
    CAS 
    PubMed 

    Google Scholar 

  • Jacob, A. et al. Differentiation of human pluripotent stem cells into functional lung alveolar epithelial cells. Cell Stem Cell 21, 472–488 (2017).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Jacob, A. et al. Derivation of self-renewing lung alveolar epithelial type II cells from human pluripotent stem cells. Nat. Protoc. 14, 3303–3332 (2019).

    Article 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar 

  • Abo, K. M. et al. Air–liquid interface culture promotes maturation and allows environmental exposure of pluripotent stem cell–derived alveolar epithelium. JCI Insight 7, e155589 (2022).

  • share Share facebook pinterest whatsapp x print

    Similar Content

    Bri McDaniel leaves with a knee injury for No. 8 Maryland against No. 24 Minnesota
    US rugby lines up busy schedule for men’s and women’s Eagles from April to July
    Marvel Rivals review – discomfitingly slick hero shooter makes you worry about gaming’s future
    Marvel Rivals review – discomfitingly slick hero shooter makes you worry about gaming’s future
    Do you need to make $100,000 to buy a home? Census data says yes
    Do you need to make $100,000 to buy a home? Census data says yes
    Dua Lipa: The 60 Minutes Interview
    Dua Lipa: The 60 Minutes Interview
    The Financial Conduct Authority
    Hedge funds push UK watchdog for a post-Brexit cut in reporting rules
    TSMC CEO is optimistic about Arizona chip quality despite challenges, most advance tech will remain in Taiwan
    TSMC CEO is optimistic about Arizona chip quality despite challenges, most advance tech will remain in Taiwan
    Uncovering the Untold: Where the Real Stories Lie | © 2025 | Daily News